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neb.com

neb is a leader in the discovery and development of molecular biology reagents. restriction enzymes, polymerases, competent cells,sample prep for ngs, and more.

thermofisher.com

Welcome to Invitrogen.com and AppliedBiosystems.com (and permutation webpages that provide a direct link to this Privacy Policy – collectively the “Sites”), which are owned and operated by Life Technologies Corporation (“Life

promega.com

Promega Corporation is a worldwide leader in applying biochemistry and molecular biology to the development of innovative, high-value products for the life sciences.

synthego.com

This tech note takes a closer look at a new high throughput method to evaluate novel nucleases leveraging Synthego's Halo™ Platform for Synthetic sgRNA and automation capabilities coupled with Paragon Genomics' ClearPlex™ amplicon sequencing technology.

nzytech.com

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baseclick.eu

Welcome to our platform dedicated to Click Chemistry protocols, specializing in CUAAC and SPAAC.

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megazyme.com

The Rapid Integrated Total Dietary Fiber Assay Kit method is validated under collaborative study (AACC Method 32-60.01, AOAC Method 2022.01, AOAC Method 2017.16, ICC Standard No. 185) and is recognized as a Type I Method by CODEX Alimentarius. The K-RINTDF method is the recommended one for the measurement of total dietary fiber in all foods that may or may not contain resistant starch. This method is updated to be more consistent with in vivo conditions in the human small intestine, i.e. a 4 h incubation time. Under these conditions more accurate measurement of resistant starch is obtained, including phosphate cross-liked starch (RS 4). Use of higher enzyme concentrations ensures that resistant maltodextrins produced from non-resistant starch under the incubation conditions of the Integrated Total Dietary Fiber procedure (AOAC Methods 2009.01 and 2011.25) are no longer produced. In this improved, rapid method, the incubation time with PAA + AMG is reduced to 4 h and the levels of both PAA and AMG are increased to ensure that resistant starch levels obtained with a set of control samples are consistent with ileostomy data. Under these conditions, the DF values obtained for most samples are the same as those obtained with AOAC Methods 2009.01 and 2011.25. The dietary fiber fractions that are measured with this method are: 1. High Molecular Weight Dietary Fiber (HMWDF) including Insoluble Dietary Fiber (IDF) and High Molecular Weight Soluble Dietary Fiber (SDFP; soluble dietary fiber which is precipitated in the presence of 78% aqueous ethanol), and 2. Low Molecular Weight Soluble Dietary Fiber (SDFS; water soluble dietary fiber that is soluble in the presence of 78% aqueous ethanol). Alternatively, IDF, SDFP and SDFS can be measured separately. The enzymes used in this method are high purity and effectively devoid of contaminating enzymes active on other dietary fiber components such as β-glucan, pectin and arabinoxylan. They are supplied as freeze-dried powders

genetex.com

we strive to serve and accelerate life science research by creating essential biomedical reagents including antibodies, proteins and research kits.