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sci-hub.se

sci-hub website. get free access to academic journals. download research papers for free from sciencedirect, ieee, wiley, springer, nature and others

scienceopen.com

Background Human lung mast cells (HLMCs) infiltrate the airway epithelium and airway smooth muscle (ASM) in asthmatic airways. The mechanism of HLMC adhesion to both cell types is only partly defined, and adhesion is not inhibited by function-blocking anti-Kit and anti-stem cell factor (SCF) antibodies. Our aim was to identify adhesion molecules expressed by human mast cells that mediate adhesion to human ASM cells (HASMCs) and human airway epithelial cells. Methods We used phage-display to isolate single chain Fv (scFv) antibodies with adhesion-blocking properties from rabbits immunised with HLMC and HMC-1 membrane proteins. Results Post-immune rabbit serum labelled HLMCs in flow cytometry and inhibited their adhesion to human BEAS-2B epithelial cells. Mast cell-specific scFvs were identified which labelled mast cells but not Jurkat cells by flow cytometry. Of these, one scFv (A1) consistently inhibited mast cell adhesion to HASMCs and BEAS-2B epithelial cells by about 30 %. A1 immunoprecipitated Kit (CD117) from HMC-1 lysates and bound to a human Kit-expressing mouse mast cell line, but did not interfere with SCF-dependent Kit signalling. Conclusion Kit contributes to human mast cell adhesion to human airway epithelial cells and HASMCs, but may utilise a previously unidentified adhesion domain that lies outside the SCF binding site. Targeting this adhesion pathway might offer a novel approach for the inhibition of mast cell interactions with structural airway cells, without detrimental effects on Kit signalling in other tissues.

oa.mg

ScholarMind enables you as a researcher to quickly extract information from your essays, research papers, and books and talk to your PDF documents as if they were human.

researchgate.net

Access 160+ million publication pages and connect with 25+ million researchers. Join for free and gain visibility by uploading your research.

datacite.org

The DataCite Distinguished Service Award recognizes individuals who have made extraordinary and sustained contributions to advancing open research, strengthening the DataCite community, and enhancing global research infrastructure. As a community-driven […]

pdfsearchengine.net

pdf search engine allows you to find free pdf books and files and download them to your computer. search through millions of online pdfs.

sciencegate.app

Springer Science analytics report presents the basic bibliometric information, impact metrics, top journals, keywords, etc. of the publisher.

journals4free.com

journals for free (j4f) provides a large database of links to free scientific journals available on the web. journals can be searched using free text and filtered by category, language and presence on other databases.

elib.dlr.de

deutsches zentrum für luft- und raumfahrt e.v., elib - dlr electronic library