genome.org Sitesinin İlk 10 Rakibi

genome.cshlp.org

an international, peer-reviewed genome sciences journal featuring outstanding original research that offers novel insights into the biology of all organisms

genomebiology.com

Background The oocyte-to-embryo transition (OET) converts terminally differentiated gametes into a totipotent embryo and is critically controlled by maternal mRNAs and proteins, while the genome is silent until zygotic genome activation. How the transcriptome, translatome, and proteome are coordinated during this critical developmental window remains poorly understood. Results Utilizing a highly sensitive and quantitative mass spectrometry approach, we obtain high-quality proteome data spanning seven mouse stages, from full-grown oocyte (FGO) to blastocyst, using 100 oocytes/embryos at each stage. Integrative analyses reveal distinct proteome reprogramming compared to that of the transcriptome or translatome. FGO to 8-cell proteomes are dominated by FGO-stockpiled proteins, while the transcriptome and translatome are more dynamic. FGO-originated proteins frequently persist to blastocyst while corresponding transcripts are already downregulated or decayed. Improved concordance between protein and translation or transcription is observed for genes starting translation upon meiotic resumption, as well as those transcribed and translated only in embryos. Concordance between protein and transcription/translation is also observed for proteins with short half-lives. We built a kinetic model that predicts protein dynamics by incorporating both initial protein abundance in FGOs and translation kinetics across developmental stages. Conclusions Through integrative analyses of datasets generated by ultrasensitive methods, our study reveals that the proteome shows distinct dynamics compared to the translatome and transcriptome during mouse OET. We propose that the remarkably stable oocyte-originated proteome may help save resources to accommodate the demanding needs of growing embryos. This study will advance our understanding of mammalian OET and the fundamental principles governing gene expression.

genesdev.org

a biweekly scientific journal publishing high-quality research in molecular biology and genetics, cancer biology, biochemistry, and related fields

nature.com

Nature is the foremost international weekly scientific journal in the world and is the flagship journal for Nature Portfolio. It publishes the finest ...

pomics.com

plant omics is a peer-reviewed scholarly journal dedicated to advancing the field of agriculture through rigorous research, innovative studies, and insightful analyses. with a commitment to excellence and a broad scope, our journal serves as a vital platform for researchers, academicians, and professionals in the agricultural sciences.

publish.csiro.au

csiro publishing, australia's leading publisher of quality scientific, technical and health journals, books, multimedia and magazines

revistaphyton.fund-romuloraggio.org.ar

phyton, international journal of experimental botany publishes original, scientific papers dealing with soil ecology and the ecology of vascular plants and bryophytes in terrestrial, aquatic and wetland ecosystems.