Les 10 concurrents genomenewsnetwork.org les plus visités

dnaftb.org

discover the concepts and experiments that define the fields of genetics and molecular biology. this animated primer features the work of over 100 scientists and researchers.

genomebiology.com

Background The oocyte-to-embryo transition (OET) converts terminally differentiated gametes into a totipotent embryo and is critically controlled by maternal mRNAs and proteins, while the genome is silent until zygotic genome activation. How the transcriptome, translatome, and proteome are coordinated during this critical developmental window remains poorly understood. Results Utilizing a highly sensitive and quantitative mass spectrometry approach, we obtain high-quality proteome data spanning seven mouse stages, from full-grown oocyte (FGO) to blastocyst, using 100 oocytes/embryos at each stage. Integrative analyses reveal distinct proteome reprogramming compared to that of the transcriptome or translatome. FGO to 8-cell proteomes are dominated by FGO-stockpiled proteins, while the transcriptome and translatome are more dynamic. FGO-originated proteins frequently persist to blastocyst while corresponding transcripts are already downregulated or decayed. Improved concordance between protein and translation or transcription is observed for genes starting translation upon meiotic resumption, as well as those transcribed and translated only in embryos. Concordance between protein and transcription/translation is also observed for proteins with short half-lives. We built a kinetic model that predicts protein dynamics by incorporating both initial protein abundance in FGOs and translation kinetics across developmental stages. Conclusions Through integrative analyses of datasets generated by ultrasensitive methods, our study reveals that the proteome shows distinct dynamics compared to the translatome and transcriptome during mouse OET. We propose that the remarkably stable oocyte-originated proteome may help save resources to accommodate the demanding needs of growing embryos. This study will advance our understanding of mammalian OET and the fundamental principles governing gene expression.

dnalc.org

dna, genetics, and biotechnology hands-on educational programs and online resources for students, educators, and the public.

yourgenome.org

Discover more about DNA, genes and genomes

genome.gov

The Genomics and Health Disparities Interest Group provides a forum to connect individuals from different scientific disciplines across the National Institutes of Health (NIH) and researchers in the Washington D.C. metropolitan area engaged in genomics and health disparities research, as well as provide opportunities for professional development, networking and community engagement.

jcvi.org

A Seamless Integration of Scientific Vision, Purpose and Technology JCVI’s commitment to environmental stewardship inspired the design and...

accessexcellence.org

a place in cyberspace for biology, health and life science teachers and learners.

completegenomics.com

Stereo-seq Technology Unbiased Capture of Whole Spatial Transcriptome Revolutionizing Spatial Omics with Nanoscale Precision Stereo-seq (Spatio-Temporal Enhanced REsolution Omics-sequencing) is a sequencing-based spatial transcriptomics technology that combines nanometer-scale resolution with an ultra-large capture area, enabling researchers to visualize gene expression across tissues with unprecedented detail and scale. It leverages DNA Nanoball (DNB)-based patterned arrays with...