DQF Fluorescent Dye – A Rhodamine-Based Dye with Large Stokes Shift Organic small-molecule fluorescent dyes have been widely applied in functional molecular labeling and detection, offering real-time, in-situ visualization for various biomedical research applications. However, most commonly used fluorescent dyes—such as fluorescein, rhodamine, oxazine, and cyanine—suffer from small Stokes shifts (typically <30 nm), leading to: Low signal-to-noise ratio, affecting imaging accuracy. Severe fluorescence self-quenching, limiting biological imaging applications. To address this limitation, the Zhang Xiaobing-Yuan Lin research group at Hunan University developed a general strategy known as Alternating Vibronic Structures (Vibration Structures) to increase the Stokes shift of conventional fluorescent dyes. This breakthrough led to the successful synthesis of DQF fluorescent dyes, a novel class of rhodamine-based dyes with enhanced optical properties.   Professor Yuan Lin is a professor and doctoral supervisor at the School of Chemistry and Chemical Engineering, Hunan University and the State Key Laboratory of Chemical Biosensing and Metrology.Currently, he has published more than 100 papers in international academic journals, with a total citation of more than 12,000 times, and an h-index of 58.Among them, the first or corresponding author from 2012 to presentJ. Am. Chem. Soc.10 articles、 Nat. Commun.1 articles、 Angew. Chem. Int. Ed.10 articles、CCS Chem.1 articles、Chem. Sci.4 articles、Anal. Chem.10 articles、Adv. Funct. Mater、BiomaterialsPublished more than 70 papers in journals and other journals. Mainly engaged in research on small molecule fluorescent probes and their applications.   Key Advantages of DQF Fluorescent Dyes Compared to traditional rhodamine dyes, DQF fluorescent dyes—including DQF-560, DQF-565, and DQF-584 Figure 1—offer: Larger Stokes shift → Reduces fluorescence self-quenching, improving imaging clarity.Better photostability → Ensures long-term fl