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bluetigerscientific.com

Blue Tiger Scientific is the one-stop shop created to outfit and provide supplies and specialty equipment dedicated for scientific labs. We have the best prices and are dedicated to quality customer service.

megazyme.com

The Total Dietary Fiber Assay Kit for the analysis of Total, Soluble and Insoluble Dietary Fiber according to AOAC and AACC approved methods. View Dietary Fiber Measurement Guide - Which Method for which sample? Dietary fiber can generally be described as the carbohydrate content of food that is not digested in the human small intestine. It passes into the large intestine where it is partially or fully fermented. These characteristics of dietary fiber are associated with its numerous well documented health benefits. Dietary Fiber is a mixture of complex organic substances, including hydrophilic compounds, such as soluble and insoluble polysaccharides and non-digestable oligosaccharides, as well as a range of non-swellable, more or less hydrophobic, compounds such as cutins, suberins and lignins. The procedures for the determination and analysis of total dietary fiber as outlined in our assay protocol are based on the methods of Lee et al. 1 and Prosky et al. 2,3 (AOAC 991.43, AOAC 985.29, AACC 32-07.01 and AACC 32-05.01). However, the enzymes in the Megazyme Total Dietary Fiber Kit can also be used in other dietary fiber analytical methods such as AACC Method 32-21.01 and AACC Method 32-06.01. 1. Association of Official Analytical Chemists. (1985). Official Methods of Analysis, 14th ed., 1st suppl. Secs. 43, A14-43, A20, p.399. 2. Association of Official Analytical Chemists. (1986). Changes in methods. J. Assoc. Off. Anal. Chem., 69, 370. 3. Association of Official Analytical Chemists. (1987). Changes in methods. J. Assoc. Off. Anal. Chem., 70, 393. See General Referee Reports: Journal of AOAC INTERNATIONAL, Vol. 81, No. 1, 1998. Two separate methods are described in the associated assay protocol: METHOD 1: DETERMINATION OF TOTAL, SOLUBLE AND INSOLUBLE DIETARY FIBER Based on AOAC Method 991.43 “Total, Soluble, and Insoluble Dietary Fiber in Foods” (First Action 1991) and AACC Method 32-07.01 “Determination of Soluble, Insoluble, and Total Dietary Fiber in Foo

leebio.com

we're leaders in biologics manufacturing, process development, and more. visit our site now to learn more about our products and customized solutions.

cellmosaic.com

Download Documents Product Sheet SDS COA_Lot#NB0350512 CellMosaic’s Phenyldiazirine sxLink™ is a proprietary photo-crosslinking reagent developed by CellMosaic® for studying biomolecular interactions. This reagent combines a highly efficient, carbene-generating phenyldiazirine group with convenient oxime-based reversible chemical crosslinking and the super-hydrophilic AqT® linker. These structural features make this reagent highly advantageous compared to traditional photo-crosslinking reagents. Trifluoromethyl phenyldiazirine (TFMP) group: TFMP photolyzes around 360 nm, at which photodamage to biomolecules is minimized. The generated carbene inserts C–H bonds into the neighboring biomolecular partner within picoseconds. Because the electron-withdrawing trifluoromethyl group confers stability on the intermediate diazo-isomer, no side products are detected under normal labeling conditions. Reversible disulfide for chemical crosslinking of any biomolecule containing a free thiol. Figure 1 shows a workflow for how a reversible sxLink™ can be used to crosslink the interacting biomolecule partner and identify the crosslinking site or detect the interaction partner. Hydrophilic AqT® linkers: TFMP group is highly hydrophobic, biomolecules labeled with a TFMP using a traditional ethylene and ethylene glycol-type linker tend to aggregate and destabilize the labeled protein. The AqT® linker greatly enhances the hydrophilicity and water solubility of sxLink™ (2.2 mg/mL). It also improves biocompatibility and reduces non-specific hydrophobic interactions with other biomolecules, allowing high loading of phenyldiazirine groups. The product is sold as a lyophilized powder in either 1 vial of 250 µg (Cat# CM81401-250UG) or 4 vials of 250 µg (Cat# CM81401-4x250UG). One vial of dissolution buffer is included in each configuration. Chemical Information C

ibl-america.com

Solid phase sandwich ELISA for the determination of human soluble Alpha Klotho (α-Klotho) in serum and EDTA-plasma. For research use only, not for use in diagnostic procedures. α-Klotho is a key player that integrates a multi-step regulatory system of calcium metabolism and phosphate homeostasis. α-klotho was identified as an extremely down-regulated gene in the genetic mutation mouse (Klotho mouse) of which phenotype is very similar to various symptoms of human aging. Then, the sequences of α-Klotho genes have been identified in various species including human based on the one of mouse. α-Klotho protein is a 130kDa, one-transmembrane protein and its expression is confirmed in the kidneys and the parathyroid glands. In recent years, it has become clear that α-Klotho is an important molecule within a living organism regulating the metabolism of mineral such as calcium and phosphorus. Therefore, it is considered that, in Klotho mouse, early aging-like symptoms are induced by impaired mineral homeostasis caused by decreased expression of α- Klotho. Meanwhile, it is reported that the long N-terminal extracellular domain which comprises the major portion of sequence of α-Klotho protein is released free into blood by shedding. However, there are may unclear points about functions and changes in concentration of free (soluble) α-Klotho protein, so it has been required to develop the measurement system of α-Klotho. Human soluble α-Klotho protein in human blood and urine can be measured by this assay kit. Control Set 27998-QC is available for purchase.

cellsciences.com

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bpcinstruments.com

BPC Instruments provides precision lab instruments for biogas, biodegradability and animal nutrition. Trusted by researchers and industry worldwide.

world-of-peptides.com

Explore the science behind GHRP and GHRH. Understand how these peptides work together to boost growth hormone levels and improve performance.

retaonelabs.com

Domestic orders typically ship within 24-48 hours and arrive within 2-5 business days. International orders may take 7-14 business days depending on customs