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pubpub.org

Collaborative Community Publishing

publicationethics.org

promoting integrity in scholarly research and its publication

eneuro.org

Recently developed probes for extracellular electrophysiological recordings have large numbers of electrodes on long linear shanks. Linear electrode arrays, such as Neuropixels probes, have hundreds of recording electrodes distributed over linear shanks that span several millimeters. Because of the length of the probes, linear probe recordings in rodents usually cover multiple brain areas. Typical studies collate recordings across several recording sessions and animals. Neurons recorded in different sessions and animals thus have to be aligned to each other and to a standardized brain coordinate system. Here, we evaluate two typical workflows for localization of individual electrodes in standardized coordinates. These workflows rely on imaging brains with fluorescent probe tracks and warping 3D image stacks to standardized brain atlases. One workflow is based on tissue clearing and selective plane illumination microscopy (SPIM), whereas the other workflow is based on serial block-face two-photon (SBF2P) microscopy. In both cases electrophysiological features are then used to anchor particular electrodes along the reconstructed tracks to specific locations in the brain atlas and therefore to specific brain structures. We performed groundtruth experiments, in which motor cortex outputs are labeled with ChR2 and a fluorescence protein. Light-evoked electrical activity and fluorescence can be independently localized. Recordings from brain regions targeted by the motor cortex reveal better than 0.1-mm accuracy for electrode localization, independent of workflow used.

peerreviewcongress.org

Enhancing the quality and credibility of science.

openbookpublishers.com

Richard Rorty (1931–2007), once dubbed ‘the man who killed truth’, is best known for challenging the idea that philosophy provides foundational knowledge. Yet beyond the controversy lies a vital, underexplored side of Rorty’s work: his constructive vision for fostering democratic solidarity in a world shaped by contingency and uncertainty. This volume shifts focus from defending Rorty to applying his insights for today’s fractured, post-truth culture.

oalib.com

open access library (www.oalib.com) is an academic search engine and publisher. you can download research papers for free and submit your paper to it. it is a shared academic database.

researchgate.net

Access 160+ million publication pages and connect with 25+ million researchers. Join for free and gain visibility by uploading your research.

sciencedirect.com

sciencedirect is the world's leading source for scientific, technical, and medical research. explore journals, books and articles.

nature.com

Nature is the foremost international weekly scientific journal in the world and is the flagship journal for Nature Portfolio. It publishes the finest ...