Top 10 postnatural.org Wettbewerber

cfz.org.uk

🎵 Enter the Strange Soundscape of Jon Downes 🎵 From the shadowy edge of folklore to the heart of the unknown, Jon Downes—cryptozoologist, author, and underground musician—invites you to explore a m…

diybio.org

Developer: Keegan Cooke Date: 18 Mar 2012 Description: Case, display, and control electronics for dremelfuge-based centrifuge. Based on GoGoBoard. Created as part of Standford FabLabs at School pro…

nig.ac.jp

国立遺伝学研究所へのお問い合わせは、下記カテゴリーから「メール」にてお願いいたします。 以下のお問い合わせにはお答えいたしかねますので、ご了承ください。 個人的な遺伝や疾病に関するご相談、検査等のご依頼 商品等の営業を目的としたお問い合わせ 研究所の業務とは関連のないお問い合わせ ...

thinkgene.com

A biology blog about genetics, genomics, and biotechnology

geneticsevolution.suite101.com

suite101 is a new way to talk about the things that move us. made in vancouver.

tycho5s.wordpress.com

society, culture and biology

genomebiology.com

Background The oocyte-to-embryo transition (OET) converts terminally differentiated gametes into a totipotent embryo and is critically controlled by maternal mRNAs and proteins, while the genome is silent until zygotic genome activation. How the transcriptome, translatome, and proteome are coordinated during this critical developmental window remains poorly understood. Results Utilizing a highly sensitive and quantitative mass spectrometry approach, we obtain high-quality proteome data spanning seven mouse stages, from full-grown oocyte (FGO) to blastocyst, using 100 oocytes/embryos at each stage. Integrative analyses reveal distinct proteome reprogramming compared to that of the transcriptome or translatome. FGO to 8-cell proteomes are dominated by FGO-stockpiled proteins, while the transcriptome and translatome are more dynamic. FGO-originated proteins frequently persist to blastocyst while corresponding transcripts are already downregulated or decayed. Improved concordance between protein and translation or transcription is observed for genes starting translation upon meiotic resumption, as well as those transcribed and translated only in embryos. Concordance between protein and transcription/translation is also observed for proteins with short half-lives. We built a kinetic model that predicts protein dynamics by incorporating both initial protein abundance in FGOs and translation kinetics across developmental stages. Conclusions Through integrative analyses of datasets generated by ultrasensitive methods, our study reveals that the proteome shows distinct dynamics compared to the translatome and transcriptome during mouse OET. We propose that the remarkably stable oocyte-originated proteome may help save resources to accommodate the demanding needs of growing embryos. This study will advance our understanding of mammalian OET and the fundamental principles governing gene expression.

dnalc.org

dna, genetics, and biotechnology hands-on educational programs and online resources for students, educators, and the public.