Топ-10 конкурентов medcraveonline.com

medcrave.com

medcrave home

jscimedcentral.com

jscimed central provides scientific medical journals & research papers open access pdf download. publish quality peer-reviewed journals in science field.

medwinpublishers.com

Although the comet assay is now a popular method to assess DNA damage induced by environmental genotoxins at a single cell level, it has not been excluded the criticism that some or all of the comet images may be the results of apoptosis- and necrosismediated DNA fragmentation. In this study, therefore, we query whether necrosis in mouse stomach and liver can lead to false positive results of in vivo comet assay and whether the validity the diffusion assay detecting cells with DNA fragmentation due to apoptosis or necrosis to exclude false positive results in in vivo comet assay of. Carbon tetrachloride (CCl4), allyl alcohol (AA), and D-galactoseamine (D-Gal) that are non-genotoxic but induce necrosis in the liver and high concentration sodium chloride solution (NaCl, 250mg/mL w/v) and dimethylamine (DMA) that are non-genotoxic but induce necrosis in the stomach mucosa were given to mice. Also, diethylnitrsoamine (DEN) and ethylmethane sulfonate (EMS) that are genotoxic were given to mice at non-cytotoxic level. In the liver and stomach mucosa, not only the alkaline comet assay but also the neutral comet and diffusion assays to detect DNA fragmentation, TUNNEL assay, and histopathological observation were conducted. The stomach mucosa of mice received NaCl and DMA and the liver of mice received AA, CCl4, and D-Gal showed necrosis, the incidence of TUNNEL-positive cells, and the increases in Halo-image in the diffusion assay but not increased tail length in the alkaline comet assay. Therefore, the possibility is not considered that the generation of DNA fragments due to cell death is responsible for an increase in tail length in the alkaline comet assay but that the increase in Halo-image well agrees with cell death observed by histopathological observation and TUNNEL staining. Therefore, if a concurrently diffusion assay performed in parallel with the alkaline comet assay is positive, the possibility could not be excluded that the positive result of the comet assay

sciencedirect.com

sciencedirect is the world's leading source for scientific, technical, and medical research. explore journals, books and articles.

pubmed.ncbi.nlm.nih.gov

Bill & Melinda Gates Foundation.

researchgate.net

Access 160+ million publication pages and connect with 25+ million researchers. Join for free and gain visibility by uploading your research.

nature.com

Nature is the foremost international weekly scientific journal in the world and is the flagship journal for Nature Portfolio. It publishes the finest ...

mdpi.com

MDPI is a publisher of peer-reviewed, open access journals since its establishment in 1996.

jnnp.bmj.com

A peer-reviewed neurology, neurosurgery and psychiatry journal publishing the latest research and reviews on the entire genre of neurological sciences.