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The K-XYLX6-1V pack size has been discontinued (read more) The XylX6 assay reagent for the measurement of endo -xylanase (endo -1,4-β-xylanase) contains two components; 1) 4,6- O -(3-Ketobutylidene)-4-nitrophenyl-β-D-4 5 -glucosyl-xylopentaoside and 2) β-xylosidase. The ketone blocking group prevents any hydrolytic action by the β-xylosidase or other exo -acting glycosidases on the XylX6 substrate. Incubation with an endo -xylanase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-xylosidase. The rate of formation of 4-nitrophenol is therefore directly related to the hydrolysis of XylX6 by the endo -xylanase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH = 10.0). Need to measure other enzyme activities? View our complete list of assay kits for measurement of enzyme activity. Note that standard curves relating the absorbance obtained using the XylX6 assay to endo -xylanase activity on the native substrates, wheat arabinoxylan and beechwood xylan, are provided in the Supporting Information file under the Documents tab.

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Solid phase sandwich ELISA for the determination of human soluble Alpha Klotho (α-Klotho) in serum and EDTA-plasma. For research use only, not for use in diagnostic procedures. α-Klotho is a key player that integrates a multi-step regulatory system of calcium metabolism and phosphate homeostasis. α-klotho was identified as an extremely down-regulated gene in the genetic mutation mouse (Klotho mouse) of which phenotype is very similar to various symptoms of human aging. Then, the sequences of α-Klotho genes have been identified in various species including human based on the one of mouse. α-Klotho protein is a 130kDa, one-transmembrane protein and its expression is confirmed in the kidneys and the parathyroid glands. In recent years, it has become clear that α-Klotho is an important molecule within a living organism regulating the metabolism of mineral such as calcium and phosphorus. Therefore, it is considered that, in Klotho mouse, early aging-like symptoms are induced by impaired mineral homeostasis caused by decreased expression of α- Klotho. Meanwhile, it is reported that the long N-terminal extracellular domain which comprises the major portion of sequence of α-Klotho protein is released free into blood by shedding. However, there are may unclear points about functions and changes in concentration of free (soluble) α-Klotho protein, so it has been required to develop the measurement system of α-Klotho. Human soluble α-Klotho protein in human blood and urine can be measured by this assay kit. Control Set 27998-QC is available for purchase.

cellsciences.com

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bpcinstruments.com

Support Whether you need help with setup, software, troubleshooting or technical documentation, our support team is here to guide you. Contact us and we will help you get the right support for your instrument and application. Contact support We’re here to help! That’s why we aim to provide responsive, practical support across the full lifecycle […]

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retaonelabs.com

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