Download Documents Product Sheet SDS COA_Lot#NB0350512 CellMosaic’s Phenyldiazirine sxLink™ is a proprietary photo-crosslinking reagent developed by CellMosaic® for studying biomolecular interactions. This reagent combines a highly efficient, carbene-generating phenyldiazirine group with convenient oxime-based reversible chemical crosslinking and the super-hydrophilic AqT® linker. These structural features make this reagent highly advantageous compared to traditional photo-crosslinking reagents. Trifluoromethyl phenyldiazirine (TFMP) group: TFMP photolyzes around 360 nm, at which photodamage to biomolecules is minimized. The generated carbene inserts C–H bonds into the neighboring biomolecular partner within picoseconds. Because the electron-withdrawing trifluoromethyl group confers stability on the intermediate diazo-isomer, no side products are detected under normal labeling conditions. Reversible disulfide for chemical crosslinking of any biomolecule containing a free thiol. Figure 1 shows a workflow for how a reversible sxLink™ can be used to crosslink the interacting biomolecule partner and identify the crosslinking site or detect the interaction partner. Hydrophilic AqT® linkers: TFMP group is highly hydrophobic, biomolecules labeled with a TFMP using a traditional ethylene and ethylene glycol-type linker tend to aggregate and destabilize the labeled protein. The AqT® linker greatly enhances the hydrophilicity and water solubility of sxLink™ (2.2 mg/mL). It also improves biocompatibility and reduces non-specific hydrophobic interactions with other biomolecules, allowing high loading of phenyldiazirine groups. The product is sold as a lyophilized powder in either 1 vial of 250 µg (Cat# CM81401-250UG) or 4 vials of 250 µg (Cat# CM81401-4x250UG). One vial of dissolution buffer is included in each configuration. Chemical Information C