postnatural.org的 10 大竞争对手

cfz.org.uk

🎵 Enter the Strange Soundscape of Jon Downes 🎵 From the shadowy edge of folklore to the heart of the unknown, Jon Downes—cryptozoologist, author, and underground musician—invites you to explore a m…

diybio.org

an institution for the DIY biologist

nig.ac.jp

国立遺伝学研究所は、1949年に文部省の研究所として設立され、木村資生博士による分子進化の中立説をはじめ、数多くの研究業績を上げてきました。1984年には、大学共同利用機関として、学術コミュニティ全体の研究を促進する役割を引き受け、1988年には、総合研究大学院大学の遺伝学専攻を受け持って、独自の大学院教育を行うように...

thinkgene.com

A biology blog about genetics, genomics, and biotechnology

geneticsevolution.suite101.com

suite101 is a new way to talk about the things that move us. made in vancouver.

tycho5s.wordpress.com

society, culture and biology

genomebiology.com

Background The oocyte-to-embryo transition (OET) converts terminally differentiated gametes into a totipotent embryo and is critically controlled by maternal mRNAs and proteins, while the genome is silent until zygotic genome activation. How the transcriptome, translatome, and proteome are coordinated during this critical developmental window remains poorly understood. Results Utilizing a highly sensitive and quantitative mass spectrometry approach, we obtain high-quality proteome data spanning seven mouse stages, from full-grown oocyte (FGO) to blastocyst, using 100 oocytes/embryos at each stage. Integrative analyses reveal distinct proteome reprogramming compared to that of the transcriptome or translatome. FGO to 8-cell proteomes are dominated by FGO-stockpiled proteins, while the transcriptome and translatome are more dynamic. FGO-originated proteins frequently persist to blastocyst while corresponding transcripts are already downregulated or decayed. Improved concordance between protein and translation or transcription is observed for genes starting translation upon meiotic resumption, as well as those transcribed and translated only in embryos. Concordance between protein and transcription/translation is also observed for proteins with short half-lives. We built a kinetic model that predicts protein dynamics by incorporating both initial protein abundance in FGOs and translation kinetics across developmental stages. Conclusions Through integrative analyses of datasets generated by ultrasensitive methods, our study reveals that the proteome shows distinct dynamics compared to the translatome and transcriptome during mouse OET. We propose that the remarkably stable oocyte-originated proteome may help save resources to accommodate the demanding needs of growing embryos. This study will advance our understanding of mammalian OET and the fundamental principles governing gene expression.

dnalc.org

dna, genetics, and biotechnology hands-on educational programs and online resources for students, educators, and the public.